Dr. Song received her Ph.D. (2014) from China Agricultural University and the National Institute of Biological Sciences (NIBS). Then, she finished her postdoctoral training (2015-2019) at the RNA Therapeutics Institute at UMass Medical School. In Oct 2019, Dr. Song joined Westlake University as an assistant professor. 

Song lab mainly focuses on studying the mechanism of liver-related diseases (i.e., liver cancer and tissue regeneration) and improving CRISPR strategies for genetic disease correction and live cell DNA imaging.

We have developed a CRISPR FISHer live imaging system that enables high-sensitivity imaging of nonrepetitive DNA in living cells through phase separation-mediated signal amplification (https://www.nature.com/articles/s41422-022-00712-z).

In addition, Dr. Song has identified MAPK regulators as crucial tumor suppressors in the liver using a genome-wide CRISPR screen; Dr. Song helped improve the understanding of CRISPR engineering. (1) Chemically modified guide RNA improved genome editing and reduced off-target activity in vitro and in vivo; (2) Adenine base editing (ABE) was applied to an adult mouse model of tyrosinemia. 

1. Lyu XY#, Deng Y#, Huang XY#, Li ZZ#, Fang GQ, Yang D, Wang FL, Kang W, Shen E.Z*, Song CQ*, CRISPR FISHer enables high-sensitivity imaging of nonrepetitive DNA in living cells through phase separation-mediated signal amplification, Cell Research, 2022. (Cover story of the journal)

2. Yang, D., Song, CQ*. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs). In: Bae, S., Song, B. (eds) Base Editors. Methods in Molecular Biology, vol 2606. 2023

3. Fu XW, Song CQ＊. Identification and Validation of Pyroptosis-Related Gene Signature to Predict Prognosis and Reveal Immune Infiltration in Hepatocellular Carcinoma. Frontiers in Cell and Developmental Biology, 08 Nov 2021, 9:748039

4. Wu S#, Li Q#, Yin C, Xue W*, Song CQ*. Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases. Theranostics, 2020 10:4374-4382

5. Song CQ#, Jiang T#, et al. Adenine base editing in an adult mouse model of tyrosinemia. Nature Biomedical Engineering. 2020. 4:125–130 (F1000)

Comments: Nature Biomedical Engineering “Correcting tyrosinemia via a point mutation”；     Nature Biomedical Engineering “Towards better base editing

6. Yin H#, Song CQ#, et al. Partial DNA-guided Cas9 enables genome editing with reduced off-target activity. Nature Chemical Biology. 2018;14(3):311-316. 

Comments: Nature Catalysis “Better guidance is cheaper”

7. Song CQ#, Li Y#, et al. Genome-Wide CRISPR Screen Identifies Regulators of Mitogen-Activated Protein Kinase as Suppressors of Liver Tumors in Mice. Gastroenterology. 2017;152(5):1161-1173.e1.

Comments: Gastroenterology “Cancer Gene Discovery in Hepatocellular Carcinoma: The CRISPR/CAS9 Accelerator”

8. Song CQ, et al. In vivo genome editing partially restores alpha1-antitrypsin in a murine model of AAT deficiency. Human Gene Therapy. 2018;29(8):853-860. (Cover of the journal) (F1000)

9. Song CQ, Xue W. CRISPR-Cas-related technologies in basic and translational liver research. Nature Reviews Gastroenterology & Hepatology. 2018;15(5):251-252. 

10. Wang D, Li J,Song CQ, et al. Cas9-mediated allelic exchange repairs compound heterozygous recessive mutations in mice. Nature Biotechnology. 2018;36(9):839-842

11. Yin H, Song CQ, et al. Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing. Nature Biotechnology. 2017;35(12):1179-1187. 

12. Yin H, Song CQ, et al. Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo. Nature Biotechnology. 2016;34(3):328-33. 

13. Shen EZ#, Song CQ#, Lin Y#, et al. Mitoflash frequency in early adulthood predicts lifespan in Caenorhabditis elegans. Nature. 2014;508(7494):128-32. (F1000)

14. Liu C#, Song CQ#, et al. pQuant improves quantitation by keeping out interfering signals and evaluating the accuracy of calculated ratios. Analytical Chemistry. 2014;86(11):5286-94. 

All the publications can be accessed through Google Scholar:

https://scholar.google.com/citations?user=SYrvfr0AAAAJ&hl=en

songchunqing@westlake.edu.cn